上海科技大学人力资源管理
ShanghaiTech University Human Resources

Rujuan Liu      Assistant Professor, PI

Institute

School of Life Science and Technology

Research Area

RNA Modification/tRNA biology

Contact Info.

liurj@@shanghaitech.edu.cn

  

  

  

Biography

  

  

2003 B.s., Anhui University, China

2009 Ph.D in Biochemistry and molecular biology, University of Science and technology of China, China

2009-2019 Research Assistant Professor(2009-2011),   Research Associate Professor(2011-2018), Research Professor(2018-2019), Institute of Biochemistry and Cell Biology, CAS, China

2006-2008 Visiting Student, University of Kentucky, USA

2011-2012 Visiting Scholar, European Molecular Biology Laboratory (EMBL), France

2017-2017 Visiting Scholar, The University of Chicago, USA

2019-present Assistant Professor(tenure-track), Research Professor, ShanghaiTech University, China

  

  

Research Interests

  

  

RNA molecules play a   key role in all cellular processes. Chemical modifications expand RNA molecules from containing the standard four nucleotides Adenosine, Guanosine,   Uridine and Cytidine to more than 130 modified nucleotides, which increase   the complexity and flexibility of RNAs.In cells, transfer RNAs (tRNAs) are the heaviest modified RNA molecules. TRNA   is the key molecule in the “adaptor hypothesis “proposed by Fransis Crick, its most classical function is involved in   protein synthesis to ensure the decoding   of genetic information. Although this core role of tRNAs has been   identified for several decades, many fundamental questions remain to be   answered in the tRNA field, such as the function and meaning of the existence   of more than 100 different chemical modifications on tRNAs, and the mechanism   of occurrences and regulation of these dynamic modifications. Furthermore,   many tRNA and tRNA derived RNA fragments have been found to function as   regulatory RNA, which play roles beyond protein translation   in many physiological and pathological processes. What’s the role of chemical   modifications on the coordination between the classical and nonclassical functions of tRNA? The defects of tRNA modifications and mutations of some tRNA   modification enzymes are directly associated with various human diseases especially neurological development/degeneration diseases and metabolic disorders, however,  little is known about the tRNA modification mechanism, and how modification   defects lead to human diseases. Our lab is focus on the molecular mechanisms   and biological functions of dynamic tRNA modifications by using tRNA-seq,   CLIP-seq, LC-MS/MS, enzymatic assays, and other technologies of biochemistry,   biophysics and cellular biology.

  

  

Selected Publications

  

  

1. Jing   Li, Hao Li, Tao Long, Han Dong, En-Duo Wang* and Ru-Juan Liu*   (2019) Archaeal NSUN6 catalyzes m5C72   modification on a wide-range of specific tRNAs.Nucleic Acids Res. 47 (4), 2041-55.   

2.Ru-Juan Liu#*,   Tao Long#, Jing LiHao LiEn-Duo Wang*2017Structural basis   for substrate binding and catalytic mechanism of a human RNA:m5C   methyltransferase NSun6.Nucleic   Acids Res. 45 (11), 6684-97.

3.Tao   Long, Jing Li, Hao Li, Mi Zhou, Xiao-Long Zhou, Ru-Juan Liu*, and En-Duo Wang* (2016) Sequence-specific and   Shape-selective RNA Recognition by the Human RNA 5-Methylcytosine   Methyltransferase NSun6.J Biol Chem.291(46), 24293-303.

4.Mi   Zhou, Tao Long, Zhi-Peng Fang, Xiao-Long Zhou, Ru-JuanLiu*, En-Duo   Wang* (2015) Identification of determinants for   tRNA substrate recognition by Escherichia coli C/U34 2'-O-methyltransferase.RNA Biol. 12(8), 900-11.

5.Ru-Juan Liu#, Tao Long#, Mi Zhou,   Xiao-Long Zhou, En-Duo Wang* (2015)   tRNA recognition by a bacterial   tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily.Nucleic Acids Res. 43(15), 7489-503.

6.Ru-Juan Liu#,   Mi Zhou#, Zhi-Peng Fang, Meng Wang, Xiao-Long Zhou and En-Duo Wang* (2013) The tRNA recognition mechanism of   the minimalist SPOUT methyltransferase, TrmL. Nucleic Acids Res.41(16),   7828-42.