Rujuan Liu Assistant Professor, PI
2003 B.s., Anhui University, China
2009 Ph.D in Biochemistry and molecular biology, University of Science and technology of China, China
2009-2019 Research Assistant Professor(2009-2011), Research Associate Professor(2011-2018), Research Professor(2018-2019), Institute of Biochemistry and Cell Biology, CAS, China
2006-2008 Visiting Student, University of Kentucky, USA
2011-2012 Visiting Scholar, European Molecular Biology Laboratory (EMBL), France
2017-2017 Visiting Scholar, The University of Chicago, USA
2019-present Assistant Professor(tenure-track), Research Professor, ShanghaiTech University, China
RNA molecules play a key role in all cellular processes. Chemical modifications expand RNA molecules from containing the standard four nucleotides Adenosine, Guanosine, Uridine and Cytidine to more than 130 modified nucleotides, which increase the complexity and flexibility of RNAs.In cells, transfer RNAs (tRNAs) are the heaviest modified RNA molecules. TRNA is the key molecule in the “adaptor hypothesis “proposed by Fransis Crick, its most classical function is involved in protein synthesis to ensure the decoding of genetic information. Although this core role of tRNAs has been identified for several decades, many fundamental questions remain to be answered in the tRNA field, such as the function and meaning of the existence of more than 100 different chemical modifications on tRNAs, and the mechanism of occurrences and regulation of these dynamic modifications. Furthermore, many tRNA and tRNA derived RNA fragments have been found to function as regulatory RNA, which play roles beyond protein translation in many physiological and pathological processes. What’s the role of chemical modifications on the coordination between the classical and nonclassical functions of tRNA? The defects of tRNA modifications and mutations of some tRNA modification enzymes are directly associated with various human diseases especially neurological development/degeneration diseases and metabolic disorders, however, little is known about the tRNA modification mechanism, and how modification defects lead to human diseases. Our lab is focus on the molecular mechanisms and biological functions of dynamic tRNA modifications by using tRNA-seq, CLIP-seq, LC-MS/MS, enzymatic assays, and other technologies of biochemistry, biophysics and cellular biology.
1. Jing Li, Hao Li, Tao Long, Han Dong, En-Duo Wang* and Ru-Juan Liu* (2019) Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs.Nucleic Acids Res. 47 (4), 2041-55.
2.Ru-Juan Liu#*, Tao Long#, Jing Li，Hao Li，En-Duo Wang*（2017）Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6.Nucleic Acids Res. 45 (11), 6684-97.
3.Tao Long, Jing Li, Hao Li, Mi Zhou, Xiao-Long Zhou, Ru-Juan Liu*, and En-Duo Wang* (2016) Sequence-specific and Shape-selective RNA Recognition by the Human RNA 5-Methylcytosine Methyltransferase NSun6.J Biol Chem.291(46), 24293-303.
4.Mi Zhou, Tao Long, Zhi-Peng Fang, Xiao-Long Zhou, Ru-JuanLiu*, En-Duo Wang* (2015) Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2'-O-methyltransferase.RNA Biol. 12(8), 900-11.
5.Ru-Juan Liu#, Tao Long#, Mi Zhou, Xiao-Long Zhou, En-Duo Wang* (2015) tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily.Nucleic Acids Res. 43(15), 7489-503.
6.Ru-Juan Liu#, Mi Zhou#, Zhi-Peng Fang, Meng Wang, Xiao-Long Zhou and En-Duo Wang* (2013) The tRNA recognition mechanism of the minimalist SPOUT methyltransferase, TrmL. Nucleic Acids Res.41(16), 7828-42.