Tong Iris Wang Assistant Professor
2004, B.Sc. in Biological science, Shandong University, China;
2011, Ph.D. in Neurobiology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China;
2011-2012, Assistant Investigator, Institute of Neuroscience, Chinese Academy for sciences, Shanghai, China;
2012-2016, Postdoctoral Research Fellow, Queensland Brain Institute, The University of Queensland, Brisbane, Australia;
2016-2019.9, ARC DECRA Research Fellow, The University of Queensland, Brisbane, Australia.2019.9 – now, Assistant Professor, School of Life Science and Technology, ShanghaiTech University, Shanghai, China
In mammalian central nervous system (CNS), neurons are the basic functional unit for information processing. The long and thin neuronal axon segment serves as both the ‘cable’ for electrical signal transduction and the ‘high-speed railway’ for material exchange. The specialized axonal cytoskeleton facilitates the efficient long-range cargo transport and maintains the structural stability of these thin axons, which are critical for CNS function. Consistently, progressive degeneration of axons have been extensively noticed in traumatic brain injury (TBI) and neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD) and motoneuron diseases (NMD) and serves as the earliest sign or the causing reason of these pathologies. However, how this vulnerability of axons is encoded in the molecular and cellular levels, and how they are triggered by genetic or mechanical factors remain elusive. This is largely due to the small caliber of CNS axons (<0.5 μm) and the dynamic movements of various types of axonal carriers, these factors make it very challenging to study how the axonal cytoskeleton and active cargo trafficking are coordinate underlying multiple physiological and pathological processes. With the recent advances in microscopy techniques and development of in vitro microfluidic assays, which separate axons from the rest of the neuronal parts, many of the previously unattainable coordination could be unraveled. We therefore aim to investigate the coordination between the axonal structure and cargo trafficking underlying the following critical functional and pathological procedures:
1) Establish the microfluid-based in vitro platform to study the underlying mechanisms of diffusive axonal injury. 2) Explore the role of long-range axonal trafficking in the process of neuronal injury and degeneration. 3) Define the functional and regulatory pathways of the periodic axonal cytoskeleton in diffusive axonal injury and degeneration. (4) Combinedly use the microfluid techniques and computational simulation to screen for the factors promote axonal regeneration.
The ultimate goal of our research is to find ways to rescue and revert the impaired subcellular changes in the axon that leads to the severe damages in many forms of neurodegenerative diseases and neural injury.
1. Wang T, Li W, Martin S, Papadopulos A, Jiang A, Shamsollahi G, Amor R, Lanoue V, Padmanabhan P, Meunier FA (2019) Radial contractility of actomyosin-ii rings facilitates cargo trafficking and maintains axonal structural stability following cargo-induced transient axonal expansion. bioRxiv.
2. Martínez-Mármol R, Mohannak N, Qian L, Wang T, Gormal RS, Ruitenberg MJ, Vanhaesebroeck B, Coulson EJ, Meunier FA.(2019) p110δ PI3-Kinase Inhibition Perturbs APP and TNFα Trafficking, Reduces Plaque Burden, Dampens Neuroinflammation, and Prevents Cognitive Decline in an Alzheimer's Disease Mouse Model. J Neurosci. 39(40):7976-7991.
3. Wang T, Martin S, Nguyen TH, Harper CB, Gormal RS, Martinez-Marmol R, Karunanithi S, Coulson EJ, Glass NR, Cooper-White JJ, van Swinderen B, Meunier FA (2016) Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB. Nature Communications. 7: p. 12976.
4. Harper CB, Papadopulos A, Martin S, Matthews DR, Morgan GP, Nguyen TH, Wang T, Nair D, Choquet D, Meunier FA (2016) Botulinum neurotoxin type-A enters a non-recycling pool of synaptic vesicles. Scientific Reports. 6: p. 19654.
5. Narayana VK, Tomatis VM, Wang T, Kvaskoff D, Meunier FA (2015) Profiling of free fatty acids using stable isotope tagging uncovers a role for saturated fatty acids in neuroexocytosis. Cell Chemical Biology. 22: p. 1552-61.
6. Wang T, Martin S, Papadopulos A, Harper CB, Mavlyutov TA, Niranjan D, Glass NR, Cooper-White JJ, Sibarita JB, Choquet D, Davletov B, Meunier FA (2015) Control of autophagosome axonal retrograde flux by presynaptic activity unveiled using botulinum neurotoxin type A. Journal of Neuroscience. 35: p. 6179-94.
Recommended by Faculty of 1000；Highlighted in Brain Research vol:1649, 143-150 (2016)。
7. Xu XH, Deng CY, Liu Y, He M, Peng J, Wang T, Yuan L, Zheng ZS, Blackshear PJ, Luo ZG (2014) MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. Cell Research. 24: p. 576-94.
8. Liu Y, Xu XH, Chen Q, Wang T, Deng CY, Song BL, Du JL, Luo ZG (2013) Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development. Nature Communications. 4: p. 2005.
9. Wang T, Liu Y, Xu XH, Deng CY, Wu KY, Zhu J, Fu XQ, He M, Luo ZG (2011) Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization. Developmental Cell. 21: p. 431-44.
Cover story, recommended byFaculty of 1000；Highlighted in Essays Biochemistry vol:53, 55-68 (2012)。
10.Wang, J., Fu, X.Q., Lei, W.L., Wang, T., Sheng, A.L., and Luo, Z.G. (2010) Nuclear factor kappaB controls acetylcholine receptor clustering at the neuromuscular junction. J Neurosci 30: p. 11104-13.
11.Zhang X, Zhu J, Yang GY, Wang QJ, Qian L, Chen YM, Chen F, Tao Y, Hu HS, Wang T, Luo ZG (2007) Dishevelled promotes axon differentiation by regulating atypical protein kinase C. Nature Cell Biology. 9: p. 743-54.
Recommended by Faculty of 1000。