上海科技大学人力资源管理
ShanghaiTech University Human Resources

Yangfei Xiang    Assistant Professor, PI

Institute

School of Life     Science and Technology

Research Area

Stem cell and     neurobiology

Contact Info.

xiangyf@@shanghaitech.edu.cn

 

 

 

Biography

 

 

Dr.   Xiang graduated from Huaihua University in 2006. He received his MS degree in   2009 and PhD degree in 2013, both from Jinan University. During his PhD   study, he was personally supported by the Fundamental Research Funds for the   Central Universities and the Funds for Excellent Doctoral Candidate of Jinan   University. He was awarded the National (China) Scholarship for Graduate   Students and the Outstanding Graduate Student of Jinan University. After his   graduation, Dr. Xiang started his postdoctoral training at the Yale Stem Cell   Center and Department of Genetics at Yale School of Medicine since August   2013 and was promoted to Associate Research Scientist there since May 2019.   Since February 2020, Dr. Xiang joined the School of Life Science and   Technology of ShanghaiTech University as a tenure-track assistant professor   and PI.

 

 

Research Interests

 

 

Almost   thousands of years ago, the human being started to quest the mysteries of the   brain, the most complex organ in the human body, yet today a soaring number   of puzzles remain to be resolved. Basing on the knowledge in current stem   cell biology, developmental biology, and many others, a novel, cutting-edge   technology-brain organoid-was discovered and has been quickly applied to   addressing multiple biological and medical questions in recent years. Brain   organoids, three-dimensional neural cultures that can recapitulate the   spatiotemporal organization and function in the brain, provide easy access to   deciphering the human brain in a dish, which otherwise could be impossible to   achieve. Further development and optimization of the system are still needed.   For instance, the structural complexity of the brain (and the whole nervous   system) calls for the generation of brain organoids with defined regional   identity, particularly for regions of direct correlation with clinical   manifestations once they deteriorate. From human pluripotent stem cells   (hESCs and hiPSCs), we have achieved regional specifications of human brain   organoids, deciphered the differential development programs in various regions   of the human brain, and applied region-specific human brain organoids for   disease modeling. By fusing distinct region-specific brain organoids, we can   reconstruct the regional interactions and connections inside the brain in a   dish. In our next journey, we will explore the human brain organoid toolkits.   By combining with more stem cell technologies, particularly genome editing,   we will apply brain organoids to investigate the etiology and therapy of   human neurological diseases. We aim to not only address fundamental questions   in human brain research but also serve the urgent needs in medicine.

 

 

Selected Publications

 

 

(# co-first author)

1.       Xiang Y (corresponding   author),   Cakir B, Park IH. Deconstructing and reconstructing the human brain with   regionally specified brain organoids. Seminars   in Cell and Developmental Biology. In revision.

2.       Cakir   B#, Xiang Y#, Tanaka Y,   Kural MH, Parent M, Kang JY, Chapeton K, Patterson B, Yuan Y, He CS, Raredon   MSB, Dengelegj J, Kim KY, Sun P, Zhong M, Lee SH, Patra P, Hyder F, Niklason   LE, Lee SH, Yoon YS, Park IH. Development   of human brain organoids with functional vascular-like system. Nat Methods. 2019 Oct 7. doi:   10.1038/s41592-019-0586-5.

3.       Xiang Y, Cakir B, Park IH. Generation of regionally specified   human brain organoids resembling thalamus development. STAR Protocols. https://doi.org/10.1016/j.xpro.2019.100001.

4.       Xiang   Y#, Tanaka Y#, Cakir B, Patterson B, Kim KY,   Sun P, Kang YJ, Zhong M, Liu X, Patra P, Lee SH, Weissman SW, Park IH.   hESC-derived thalamic organoids form reciprocal projections when fused with   cortical organoids. Cell Stem Cell.   2019 Mar 7;24(3):487-497.

5.       Xiang   Y, Tanaka Y, Patterson B, Cakir B, Kim KY, Cho   YS, Park IH. Generation and fusion of human cortical and medial ganglionic   eminence brain organoids. Current   Protocols in Stem Cell Biology. 2018 Sep 11; doi: 10.1002/cpsc.61.

6.       Kim KY#, Tanaka Y#, Su J,   Cakir B, Xiang Y, Patterson   B, Ding JJ, Jung YW, Kim JH, Hysolli E, Lee H, Dajani R, Kim J, Zhong M, Lee   JH, Skalnik D, Lim JM, Sullivan G, Wang J, Park IH. Uhrf1 regulates active   transcriptional marks at bivalent domains in pluripotent stem cells through   Setd1a. Nature Communications.   2018 Jul 3;9(1):2583. doi: 10.1038/s41467-018-04818-0.

7.       Xiang   Y#, Tanaka Y#, Patterson B, Kang YJ, Govindaiah   G, Roselaar N, Cakir B, Kim KY, Lombroso AP, Hwang SM, Zhong M, Stanley EG,   Elefanty AG, Naegele JR, Lee SH, Weissman SM, Park IH. Fusion of regionally   specified hPSC-Derived organoids models human brain development and   interneuron migration. Cell Stem   Cell. 2017 Sep 7;21(3):383-398.e7.

8.       Xiang   Y#, Kim KY#, Gelernter J, Park IH, Zhang H.   Ethanol upregulates NMDA receptor subunit gene expression in human embryonic   stem cell-derived cortical neurons. PLoS   One. 2015 Aug 12;10(8):e0134907.

9.       Tanaka Y#, Hysolli E#, Su J,   Xiang Y, Kim KY, Zhong M,   Li Y, Heydari K, Euskirchen G, Snyder MP, Pan X, Weissman SM, Park IH.   Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming. Stem Cell Reports. 2015 Jun   9;4(6):1125-39.

10.    Xiang   Y#, Zheng K#, Zhong M, Chen J, Wang X, Wang Q,   Wang S, Ren Z, Fan J, Wang Y. Ubiquitin-proteasome-dependent slingshot 1   downregulation in neuronal cells inactivates cofilin to facilitate HSV-1   replication. Virology. 2014   Jan 20;449:88-95.

11.    Ge H#, Liu G#, Xiang Y#, Wang Y, Guo CW, Chen   NH, Zhang YJ, Wang YF, Kitazato K, Xu J. The mechanism of   poly-galloyl-glucoses preventing Influenza A virus entry into host cells. PLoS One. 2014 Apr   9;9(4):e94392.

12.    Zhong MG#, Xiang Y#, Qiu XX, Liu Z,   Kitazato K, Wang YF. Natural products as a source of anti- herpes simplex   virus agents. RSC Advances.   2013; 3:313-328

13.    Zeng FL#, Xiang Y#, Liang ZR, Wang X,   Huang DE, Zhu SN, Li MM, Yang DP, Wang DM, Wang YF. Anti-hepatitis B virus   effects of dehydrocheilanthifoline from Corydalis saxicola. Am J Chin Med.   2013;41(1):119-30.

14.    Xiang   Y, Zheng K, Ju H, Wang S, Pei Y, Ding W, Chen   Z, Wang Q, Qiu X, Zhong M, Zeng F, Ren Z, Qian C, Liu G, Kitazato K, Wang Y.   Cofilin 1-mediated biphasic F-actin dynamics of neuronal cells affect herpes   simplex virus 1 infection and replication. J Virol. 2012 Aug;86(16):8440-51.

15.    Xiang   Y#, Qian CW#, Xing GW, Hao J, Xia M, Wang YF.   Anti-herpes simplex virus efficacies of 2-aminobenzamide derivatives as novel   HSP90 inhibitors. Bioorg Med Chem   Lett. 2012 Jul 15;22(14):4703-6.

16.    Lu JX#, Xiang Y#, Zhang JX, Ju HQ, Chen ZP, Wang QL, Chen W, Peng   XL, Han B, Wang YF. Cloning, soluble expression, rapid purification and   characterization of human Cofilin1. Protein   Expr Purif. 2012 Mar;82(1):186-91.

17.    Xiang   Y, Pei Y, Qu C, Lai Z, Ren Z, Yang K, Xiong S,   Zhang Y, Yang C, Wang D, Liu Q, Kitazato K, Wang Y. In vitro anti-herpes   simplex virus activity of 1,2,4,6-tetra-O-galloyl-β-D-glucose from   Phyllanthus emblica L. (Euphorbiaceae). Phytother   Res. 2011 Jul;25(7):975-82.

18.    Ju HQ#, Xiang Y#, Xin BJ, Pei Y, Lu JX, Wang QL, Xia M, Qian CW,   Ren Z, Wang SY, Wang YF, Xing GW. Synthesis and in vitro anti-HSV-1 activity   of a novel Hsp90 inhibitor BJ-B11. Bioorg   Med Chem Lett. 2011 Mar 15;21(6):1675-7.

19.    Xiang   Y, Ju HQ, Li S, Zhang YJ, Yang CR, Wang YF.   Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and   HBeAg secretion in HepG2.2.15 cell culture. Virol Sin. 2010 Oct;25(5):375-80.