Yangfei Xiang Assistant Professor, PI
Dr. Xiang graduated from Huaihua University in 2006. He received his MS degree in 2009 and PhD degree in 2013, both from Jinan University. During his PhD study, he was personally supported by the Fundamental Research Funds for the Central Universities and the Funds for Excellent Doctoral Candidate of Jinan University. He was awarded the National (China) Scholarship for Graduate Students and the Outstanding Graduate Student of Jinan University. After his graduation, Dr. Xiang started his postdoctoral training at the Yale Stem Cell Center and Department of Genetics at Yale School of Medicine since August 2013 and was promoted to Associate Research Scientist there since May 2019. Since February 2020, Dr. Xiang joined the School of Life Science and Technology of ShanghaiTech University as a tenure-track assistant professor and PI.
Almost thousands of years ago, the human being started to quest the mysteries of the brain, the most complex organ in the human body, yet today a soaring number of puzzles remain to be resolved. Basing on the knowledge in current stem cell biology, developmental biology, and many others, a novel, cutting-edge technology-brain organoid-was discovered and has been quickly applied to addressing multiple biological and medical questions in recent years. Brain organoids, three-dimensional neural cultures that can recapitulate the spatiotemporal organization and function in the brain, provide easy access to deciphering the human brain in a dish, which otherwise could be impossible to achieve. Further development and optimization of the system are still needed. For instance, the structural complexity of the brain (and the whole nervous system) calls for the generation of brain organoids with defined regional identity, particularly for regions of direct correlation with clinical manifestations once they deteriorate. From human pluripotent stem cells (hESCs and hiPSCs), we have achieved regional specifications of human brain organoids, deciphered the differential development programs in various regions of the human brain, and applied region-specific human brain organoids for disease modeling. By fusing distinct region-specific brain organoids, we can reconstruct the regional interactions and connections inside the brain in a dish. In our next journey, we will explore the human brain organoid toolkits. By combining with more stem cell technologies, particularly genome editing, we will apply brain organoids to investigate the etiology and therapy of human neurological diseases. We aim to not only address fundamental questions in human brain research but also serve the urgent needs in medicine.
(# co-first author)
1. Xiang Y (corresponding author), Cakir B, Park IH. Deconstructing and reconstructing the human brain with regionally specified brain organoids. Seminars in Cell and Developmental Biology. In revision.
2. Cakir B#, Xiang Y#, Tanaka Y, Kural MH, Parent M, Kang JY, Chapeton K, Patterson B, Yuan Y, He CS, Raredon MSB, Dengelegj J, Kim KY, Sun P, Zhong M, Lee SH, Patra P, Hyder F, Niklason LE, Lee SH, Yoon YS, Park IH. Development of human brain organoids with functional vascular-like system. Nat Methods. 2019 Oct 7. doi: 10.1038/s41592-019-0586-5.
3. Xiang Y, Cakir B, Park IH. Generation of regionally specified human brain organoids resembling thalamus development. STAR Protocols. https://doi.org/10.1016/j.xpro.2019.100001.
4. Xiang Y#, Tanaka Y#, Cakir B, Patterson B, Kim KY, Sun P, Kang YJ, Zhong M, Liu X, Patra P, Lee SH, Weissman SW, Park IH. hESC-derived thalamic organoids form reciprocal projections when fused with cortical organoids. Cell Stem Cell. 2019 Mar 7;24(3):487-497.
5. Xiang Y, Tanaka Y, Patterson B, Cakir B, Kim KY, Cho YS, Park IH. Generation and fusion of human cortical and medial ganglionic eminence brain organoids. Current Protocols in Stem Cell Biology. 2018 Sep 11; doi: 10.1002/cpsc.61.
6. Kim KY#, Tanaka Y#, Su J, Cakir B, Xiang Y, Patterson B, Ding JJ, Jung YW, Kim JH, Hysolli E, Lee H, Dajani R, Kim J, Zhong M, Lee JH, Skalnik D, Lim JM, Sullivan G, Wang J, Park IH. Uhrf1 regulates active transcriptional marks at bivalent domains in pluripotent stem cells through Setd1a. Nature Communications. 2018 Jul 3;9(1):2583. doi: 10.1038/s41467-018-04818-0.
7. Xiang Y#, Tanaka Y#, Patterson B, Kang YJ, Govindaiah G, Roselaar N, Cakir B, Kim KY, Lombroso AP, Hwang SM, Zhong M, Stanley EG, Elefanty AG, Naegele JR, Lee SH, Weissman SM, Park IH. Fusion of regionally specified hPSC-Derived organoids models human brain development and interneuron migration. Cell Stem Cell. 2017 Sep 7;21(3):383-398.e7.
8. Xiang Y#, Kim KY#, Gelernter J, Park IH, Zhang H. Ethanol upregulates NMDA receptor subunit gene expression in human embryonic stem cell-derived cortical neurons. PLoS One. 2015 Aug 12;10(8):e0134907.
9. Tanaka Y#, Hysolli E#, Su J, Xiang Y, Kim KY, Zhong M, Li Y, Heydari K, Euskirchen G, Snyder MP, Pan X, Weissman SM, Park IH. Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming. Stem Cell Reports. 2015 Jun 9;4(6):1125-39.
10. Xiang Y#, Zheng K#, Zhong M, Chen J, Wang X, Wang Q, Wang S, Ren Z, Fan J, Wang Y. Ubiquitin-proteasome-dependent slingshot 1 downregulation in neuronal cells inactivates cofilin to facilitate HSV-1 replication. Virology. 2014 Jan 20;449:88-95.
11. Ge H#, Liu G#, Xiang Y#, Wang Y, Guo CW, Chen NH, Zhang YJ, Wang YF, Kitazato K, Xu J. The mechanism of poly-galloyl-glucoses preventing Influenza A virus entry into host cells. PLoS One. 2014 Apr 9;9(4):e94392.
12. Zhong MG#, Xiang Y#, Qiu XX, Liu Z, Kitazato K, Wang YF. Natural products as a source of anti- herpes simplex virus agents. RSC Advances. 2013; 3:313-328
13. Zeng FL#, Xiang Y#, Liang ZR, Wang X, Huang DE, Zhu SN, Li MM, Yang DP, Wang DM, Wang YF. Anti-hepatitis B virus effects of dehydrocheilanthifoline from Corydalis saxicola. Am J Chin Med. 2013;41(1):119-30.
14. Xiang Y, Zheng K, Ju H, Wang S, Pei Y, Ding W, Chen Z, Wang Q, Qiu X, Zhong M, Zeng F, Ren Z, Qian C, Liu G, Kitazato K, Wang Y. Cofilin 1-mediated biphasic F-actin dynamics of neuronal cells affect herpes simplex virus 1 infection and replication. J Virol. 2012 Aug;86(16):8440-51.
15. Xiang Y#, Qian CW#, Xing GW, Hao J, Xia M, Wang YF. Anti-herpes simplex virus efficacies of 2-aminobenzamide derivatives as novel HSP90 inhibitors. Bioorg Med Chem Lett. 2012 Jul 15;22(14):4703-6.
16. Lu JX#, Xiang Y#, Zhang JX, Ju HQ, Chen ZP, Wang QL, Chen W, Peng XL, Han B, Wang YF. Cloning, soluble expression, rapid purification and characterization of human Cofilin1. Protein Expr Purif. 2012 Mar;82(1):186-91.
17. Xiang Y, Pei Y, Qu C, Lai Z, Ren Z, Yang K, Xiong S, Zhang Y, Yang C, Wang D, Liu Q, Kitazato K, Wang Y. In vitro anti-herpes simplex virus activity of 1,2,4,6-tetra-O-galloyl-β-D-glucose from Phyllanthus emblica L. (Euphorbiaceae). Phytother Res. 2011 Jul;25(7):975-82.
18. Ju HQ#, Xiang Y#, Xin BJ, Pei Y, Lu JX, Wang QL, Xia M, Qian CW, Ren Z, Wang SY, Wang YF, Xing GW. Synthesis and in vitro anti-HSV-1 activity of a novel Hsp90 inhibitor BJ-B11. Bioorg Med Chem Lett. 2011 Mar 15;21(6):1675-7.
19. Xiang Y, Ju HQ, Li S, Zhang YJ, Yang CR, Wang YF. Effects of 1,2,4,6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg secretion in HepG2.2.15 cell culture. Virol Sin. 2010 Oct;25(5):375-80.