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| 个人简介 |  |
| 2000年9月-2004年7月，安徽农业大学，学士 2004年9月-2007年6月，安徽农业大学，硕士 2010年10月-2013年9月，德国柏林工业大学，博士 2007年7月-2010年9月，安徽农业大学，教师 2013年12月-2017年8月，美国西北大学，博士后 2017年9月至今，上海科技大学，物质科学与技术学院，助理教授、研究员 |
| 主要研究内容 | |
| 研究组主要利用无细胞合成生物学（Cell-free Synthetic Biology）等技术手段开展在蛋白、药物及健康等方面的研究工作。主要研究内容包括：1. 无细胞生物合成具有重要工业和药用价值的蛋白质；2. 新型无细胞蛋白合成体系的开发和利用；3. 天然产物药物（如非核糖体多肽类抗生素）的生物合成、发现与开发；4. 新型蛋白质生物材料的设计、开发与应用。 |
| 代表性论文 | |
| (*Corresponding author, #Co-first author) 1. Li J, Wang H, Kwon YC, Jewett MC. Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering, 2017, 114, 1343-1353 (Video highlighted) 2. Goering AW#, Li J#, McClure RA, Thomson RJ, Jewett MC, Kelleher NL. In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis. ACS Synthetic Biology, 2017, 6, 39–44 3. Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Rosenzweig AC, Jewett MC. Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology Journal, 2016, 11, 212–218 (Front cover) 4. Moatsou D#, Li J#, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK. Self-assembly of temperature-responsive protein-polymer bioconjugates. Bioconjugate Chemistry, 2015, 26, 1890–1899 (Front cover) 5. Li J*, Jaitzig J, Lu P, Süssmuth RD, Neubauer P. Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations. Microbial Cell Factories, 2015, 14, 83 6. Li J, Zhao Z, Mo T, Wang L, Li P. Immobilization of aminoacylase on electrospun nanofibrous membrane for the resolution of DL-theanine. Journal of Molecular Catalysis B: Enzymatic, 2015, 116, 24–28 7. Li J*, Jaitzig J, Theuer L, Legala OE, Süssmuth RD, Neubauer P. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. Journal of Biotechnology, 2015, 193, 16–22 8. Li J*, Neubauer P. Escherichia coli as a cell factory for heterologous production of nonribosomal peptides and polyketides. New Biotechnology, 2014, 31, 579–585 (Invited review) 9. Li J, Jaitzig J, Hillig F, Süssmuth RD, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Applied Microbiology and Biotechnology, 2014, 98, 591–601 10. Jaitzig J#, Li J#, Süssmuth RD, Neubauer P. Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli. ACS Synthetic Biology, 2014, 3, 432–438 11. Li J, Li P, Liu F. Production of theanine by Xerocomus badius (mushroom) using submerged fermentation. LWT-Food Science and Technology, 2008, 41, 883–889 12. Li P, Wan X, Zhang Z, Li J, Shen Z. A novel assay method for theanine synthetase activity by capillary electrophoresis. Journal of Chromatography B, 2005, 819, 81–84 13. Li P, Li J, Wu C, Wu Q, Li J. Synergistic antibacterial effects of β-lactam antibiotic combined with silver nanoparticles. Nanotechnology, 2005, 16, 1912–1917 |
